# NAD+ – 500mg/vial — Research Use Only

## NAD+ – 500mg/vial

### Compare NAD+ Sizes

Same compound, three vial sizes. Larger vials carry a meaningfully lower cost per mg.

| Product | Concentration | Price | Price / mg |
| --- | --- | --- | --- |
| NAD+ 100mg | 100mg/vial | $39.00 | $0.39 |
| NAD+ 500mg | 500mg/vial | $65.00 | $0.13 |
| NAD+ 1,000mgLowest $/mg | 1,000mg/vial | $95.00 | $0.095 |

Backed by the Research [Read: Why Researchers Study NAD+ →](https://portaltowellness.net/research/nad-plus-benefits-research/)

Nicotinamide adenine dinucleotide (NAD+) is an endogenous dinucleotide coenzyme found in all living cells, functioning as a central electron carrier in mitochondrial energy metabolism. Its molecular weight is approximately 663 Da (free acid form). NAD+ exists in oxidized (NAD+) and reduced (NADH) forms and serves as a required substrate for sirtuins, PARPs, and CD38/CD157 ectoenzymes. This vial contains 500mg and is intended for laboratory and research applications.

### NAD+ and the Electron Transport Chain

NAD+ is a direct cofactor in the mitochondrial electron transport chain — every NADH molecule oxidized back to NAD+ feeds Complex I, the entry point for ATP production. As NAD+ pools decline, models of mitochondrial energy output decline with them, which is why [NAD+ availability](https://portaltowellness.net/nad-plus-research-overview-coenzyme/) is one of the most heavily studied variables in cellular-energy and aging research.

### Research Background

Published laboratory research has extensively examined NAD+ in the context of cellular bioenergetics, mitochondrial function, and aging biology. Investigators have studied NAD+ metabolism and its relationship to sirtuin deacetylase activity in cell culture and animal models. Research has explored the role of NAD+ as a substrate for poly(ADP-ribose) polymerases (PARPs) in DNA damage response studies. Published in vivo studies have examined NAD+ precursor supplementation effects on tissue NAD+ levels and metabolic parameters in rodent models. Research using cell-free and cell-based systems has also investigated NAD+-dependent signaling in models of cellular energy regulation and senescence.

### Technical Specifications

| **Compound** | β-Nicotinamide adenine dinucleotide (oxidized) |
| --- | --- |
| **Molecular Weight** | ~663 Da |
| **Purity** | ≥98% (supplier specification) |
| **Form** | Lyophilized powder |
| **Vial Size** | 500mg |
| **Storage** | −4 °F long-term |

*For research use only. Not intended for human consumption, diagnosis, treatment, or prevention of any disease or condition.*

Not sure which compounds fit your research? [Browse the Research Hub →](https://portaltowellness.net/research/)

## Frequently Asked Questions — NAD+

### What is NAD+ and why is it central to cellular energy and aging research?

Nicotinamide adenine dinucleotide (NAD+) is a coenzyme present in all living cells, functioning as an essential electron carrier in the mitochondrial electron transport chain. It is also a required substrate for sirtuins (SIRT1–7), the deacetylase enzymes central to longevity research, and for PARP enzymes involved in DNA repair. NAD+ levels decline with age — as reported in several human and animal tissues — making NAD+ precursor and direct NAD+ research a major focus in the cellular-energy and longevity fields.

### How does NAD+ activate sirtuin pathways?

Sirtuins require NAD+ as a co-substrate to function — they cannot operate without adequate NAD+ availability. When NAD+ levels are sufficient, SIRT1 deacetylates PGC-1α (driving mitochondrial biogenesis), SIRT3 maintains mitochondrial protein homeostasis, and SIRT6 supports DNA repair fidelity. Low NAD+ concentrations effectively silence these pathways. This mechanism is why sirtuin activation and NAD+ research are inseparable in longevity science.

### Why are there three NAD+ vial sizes?

Vial size is a quantity of material, not a stage in a programme. All three contain the same compound. Portal to Wellness does not publish protocols, combinations or quantities, and nothing on this page is a recommendation for use.

### What is the difference between NAD+, NMN, and NR as research compounds?

NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) are NAD+ precursors that are converted to NAD+ intracellularly after absorption. How extracellular NAD+ reaches the intracellular pool is itself an open research question.

### What has published NAD+ research examined?

Published research has examined NAD+ metabolism in cell, animal and small human studies. Portal to Wellness does not publish amounts, schedules or administration routes for any compound. This product is not for human use.

### Related research (educational)

- [Why Researchers Study NAD+: Energy, Longevity & the Hallmarks of Aging](https://portaltowellness.net/research/nad-plus-benefits-research/)
- [What Is NAD+? The Longevity Coenzyme Explained](https://portaltowellness.net/research/what-is-nad-plus/)

Research Library

- [Overlapping Pathways in Circadian, Repair and Mitochondrial Biology](https://portaltowellness.net/research/sleep-repair-energy-one-system/)
- [The Enzymes Behind NAD+ Decline](https://portaltowellness.net/research/nad-decline-cd38-nampt-research/)
- [What Determines Regulatory Status](https://portaltowellness.net/research/what-determines-regulatory-status/)
- [FDA Warning Letters Hit GLP-1 Telehealth Marketing: What the 2026 Crackdown Means](https://portaltowellness.net/research/fda-warning-letters-glp1-telehealth-crackdown/)
- [Compounded Peptide vs Research Peptide](https://portaltowellness.net/research/compounded-peptide-vs-research-peptide/)
- [Biomarkers vs Clinical Outcomes in Research](https://portaltowellness.net/research/biomarkers-vs-clinical-outcomes-research/)
- [Mitochondrial Peptide Comparison: SS-31, MOTS-c, and NAD+](https://portaltowellness.net/research/mitochondrial-peptide-comparison-ss31-mots-c-nad/)

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Source: https://portaltowellness.net/shop/nad-plus/
Research use only. Terms: https://portaltowellness.net/research-use-only/