NAD+ (nicotinamide adenine dinucleotide) is a coenzyme in mitochondrial oxidative phosphorylation and a substrate for sirtuins (SIRT1–7) and PARP enzymes. Tissue NAD+ levels have been reported to decline with age, which is why NAD+ metabolism is studied in ageing and metabolic research.

Glowing blue molecular structure representing NAD+ cellular energy pathway - Portal to Wellness

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NAD+ – 500mg/vial — Research Use Only

Name: NAD+ – 500mg/vial — Research Use Only
Brand: Portal to Wellness
SKU: NAD500-BUNDLE
Price: 65 USD
Availability: InStock

You save $11.47

NAD+ — the coenzyme central to cellular-energy research. ≥98% purity. Research use only.

Availability: In stock

Volume pricing
3–4 vials — 5% off · 5–9 vials — 10% off · 10+ vials — 15% off
Applied automatically in the cart. Combines with a discount code up to 20% off in total, and stacks on top of the subscription discount.

Research Disclaimer: All products currently listed on this site are for research purposes only.

Research Grade ≥98% purity standard

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Most reordered mitochondrial & longevity product

Why Researchers Explore NAD+ – 500mg/vial — Research Use Only

NAD+ – 500mg/vial

Compare NAD+ Sizes

Same compound, three vial sizes. Larger vials carry a meaningfully lower cost per mg.

Product	Concentration	Price	Price / mg
NAD+ 100mg	100mg/vial	$39.00	$0.39
NAD+ 500mg	500mg/vial	$65.00	$0.13
NAD+ 1,000mgLowest $/mg	1,000mg/vial	$95.00	$0.095

Backed by the ResearchRead: Why Researchers Study NAD+ →

Nicotinamide adenine dinucleotide (NAD+) is an endogenous dinucleotide coenzyme found in all living cells, functioning as a central electron carrier in mitochondrial energy metabolism. Its molecular weight is approximately 663 Da (free acid form). NAD+ exists in oxidized (NAD+) and reduced (NADH) forms and serves as a required substrate for sirtuins, PARPs, and CD38/CD157 ectoenzymes. This vial contains 500mg and is intended for laboratory and research applications.

NAD+ and the Electron Transport Chain

NAD+ is a direct cofactor in the mitochondrial electron transport chain — every NADH molecule oxidized back to NAD+ feeds Complex I, the entry point for ATP production. As NAD+ pools decline, models of mitochondrial energy output decline with them, which is why NAD+ availability is one of the most heavily studied variables in cellular-energy and aging research.

Research Background

Published laboratory research has extensively examined NAD+ in the context of cellular bioenergetics, mitochondrial function, and aging biology. Investigators have studied NAD+ metabolism and its relationship to sirtuin deacetylase activity in cell culture and animal models. Research has explored the role of NAD+ as a substrate for poly(ADP-ribose) polymerases (PARPs) in DNA damage response studies. Published in vivo studies have examined NAD+ precursor supplementation effects on tissue NAD+ levels and metabolic parameters in rodent models. Research using cell-free and cell-based systems has also investigated NAD+-dependent signaling in models of cellular energy regulation and senescence.

Technical Specifications

Compound	β-Nicotinamide adenine dinucleotide (oxidized)
Molecular Weight	~663 Da
Purity	≥98% (supplier specification)
Form	Lyophilized powder
Vial Size	500mg
Storage	−4 °F long-term

For research use only. Not intended for human consumption, diagnosis, treatment, or prevention of any disease or condition.

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Frequently Asked Questions — NAD+

What is NAD+ and why is it central to cellular energy and aging research?

Nicotinamide adenine dinucleotide (NAD+) is a coenzyme present in all living cells, functioning as an essential electron carrier in the mitochondrial electron transport chain. It is also a required substrate for sirtuins (SIRT1–7), the deacetylase enzymes central to longevity research, and for PARP enzymes involved in DNA repair. NAD+ levels decline with age — as reported in several human and animal tissues — making NAD+ precursor and direct NAD+ research a major focus in the cellular-energy and longevity fields.

How does NAD+ activate sirtuin pathways?

Sirtuins require NAD+ as a co-substrate to function — they cannot operate without adequate NAD+ availability. When NAD+ levels are sufficient, SIRT1 deacetylates PGC-1α (driving mitochondrial biogenesis), SIRT3 maintains mitochondrial protein homeostasis, and SIRT6 supports DNA repair fidelity. Low NAD+ concentrations effectively silence these pathways. This mechanism is why sirtuin activation and NAD+ research are inseparable in longevity science.

Why are there three NAD+ vial sizes?

Vial size is a quantity of material, not a stage in a programme. All three contain the same compound. Portal to Wellness does not publish protocols, combinations or quantities, and nothing on this page is a recommendation for use.

What is the difference between NAD+, NMN, and NR as research compounds?

NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) are NAD+ precursors that are converted to NAD+ intracellularly after absorption. How extracellular NAD+ reaches the intracellular pool is itself an open research question.

What has published NAD+ research examined?

Published research has examined NAD+ metabolism in cell, animal and small human studies. Portal to Wellness does not publish amounts, schedules or administration routes for any compound. This product is not for human use.